Изворни код странице Proteinska O-GlcNAkaza

{{Infobox Enzim-lat
| Name = Proteinska O--{GlcNAkaza}-
| EC_number = 3.2.1.169
| CAS_number = 
| IUBMB_EC_number = 3/2/1/169
| GO_code = 
| image = 
| width = 
| caption =
}}
'''Proteinska O--{GlcNAkaza}-''' ({{EC broj-lat|3.2.1.169}}, ''OGA'', ''glikozidna hidrolaza O--{GlcNAkaza}-'', -{''O-GlcNAkaza'', ''BtGH84''}-, ''O--{GlcNAc}- hidrolaza'') je [[enzim]] sa sistematskim imenom ''(protein)-3-O-(-{N}--acetil--{D}--glukozaminil)--{L}--serin/treonin -{N}--acetilglukozaminil hidrolaza''.<ref>{{cite journal | title = Dynamic ''O''-glycosylation of nuclear and cytosolic proteins: cloning and characterization of a neutral, cytosolic &beta;-''N''-acetylglucosaminidase from  human brain |author = Gao, Y., Wells, L., Comer, F.I., Parker, G.J. and Hart, G.W. |journal = J. Biol. Chem. |date = 2001 |volume = 276 |pages = 9838-9845 |pmid = 11148210}}</ref><ref>{{cite journal | title = Dynamic ''O''-glycosylation of nuclear and cytosolic proteins: further characterization of the nucleocytoplasmic &beta;-''N''-acetylglucosaminidase,  ''O''-GlcNAcase |author = Wells, L., Gao, Y., Mahoney, J.A., Vosseller, K., Chen, C., Rosen, A. and Hart, G.W. |journal = J. Biol. Chem. |date = 2002 |volume = 277 |pages = 1755-1761 |pmid = 11788610}}</ref><ref>{{cite journal | title = Identification of Asp<sup>174</sup> and Asp<sup>175</sup> as the key catalytic residues of human ''O''-GlcNAcase by functional analysis of site-directed mutants |author = Cetinbas, N., Macauley, M.S., Stubbs, K.A., Drapala, R. and Vocadlo, D.J. |journal = Biochemistry |date = 2006 |volume = 45 |pages = 3835-3844 |pmid = 16533067}}</ref><ref>{{cite journal | title = Structure and mechanism of a bacterial &beta;-glucosaminidase having ''O''-GlcNAcase activity |author = Dennis, R.J., Taylor, E.J., Macauley, M.S., Stubbs, K.A., Turkenburg, J.P., Hart, S.J., Black, G.N., Vocadlo, D.J. and Davies, G.J. |journal = Nat. Struct. Mol. Biol. |date = 2006 |volume = 13 |pages = 365-371 |pmid = 16565725}}</ref><ref>{{cite journal | title = Enzymatic characterization of ''O''-GlcNAcase isoforms using a fluorogenic GlcNAc substrate |author = Kim, E.J., Kang, D.O., Love, D.C. and Hanover, J.A. |journal = Carbohydr. Res. |date = 2006 |volume = 341 |pages = 971-982 |pmid = 16584714}}</ref><ref>{{cite journal | title = Purification and characterization of an ''O''-GlcNAc selective ''N''-acetyl-&beta;-D-glucosaminidase from rat spleen cytosol |author1=Dong, D.L.  |author2=Hart, G.W.  |lastauthoramp=yes |journal = J. Biol. Chem. |date = 1994 |volume = 269 |pages = 19321-19330 |pmid = 8034696}}</ref> Ovaj enzim [[Катализа|katalizuje]] sledeću [[hemijska reakcija|hemijsku reakciju]]

: (1) [protein]-3-O-(-{N}--acetil-beta--{D}--glukozaminil)--{L}--serin + -{H}-<sub>2</sub>O <math>\rightleftharpoons</math> [protein]--{L}--serin + -{N}--acetil--{D}--glukozamin
:(2) [protein]-3-O-(-{N}--acetil-beta--{D}--glukozaminil)--{L}--treonin + -{H}-<sub>2</sub>O <math>\rightleftharpoons</math> [protein]--{L}--treonin + -{N}--acetil--{D}--glukozamin

Kod viših eukariota posttranslaciona modifikacija proteinskih [[serin]]a/[[treonin]]a sa -{N}--acetilglukozaminom (O--{GlcNAc}-) je dinamička, induktivna i izobilna. Njome se regulišu mnogi ćelijski procesi putem ometanja proteinske fosforilacije.

== Reference ==
{{reflist}}

== Literatura ==
{{Литература}}
* {{PriceEnzymology3rd}}
* {{TooneAdvancesInEnzymology}}
* {{Branden ProteinStructure2nd}}
* {{SegelEnzymeKinetics}}
{{Литература крај}}
== Spoljašnje veze ==
* {{MeshName|Protein+O-GlcNAcase}}

{{Enzimi-lat}}
{{Portal bar-lat|Molekularna i ćelijska biologija|Hemija}}

{{normativna kontrola}}

[[Категорија:ЕЦ 3.2.1]]