Изворни код странице PI3K

{{Pfam_box-lat
| Symbol = -{PI3 PI4}- kinaza
| Name = Fosfatidilinozitol 3- i 4-kinaza
| image = PI3kinase.png
| width =
| caption = -{PI3}- Kinaza 110 gama vezana za inhibitor -{PIK-93}- (zut).<ref name="pmid16647110">{{PDB|2chz}}; {{cite journal |vauthors=Knight ZA, Gonzalez B, Feldman ME, Zunder ER, Goldenberg DD, Williams O, Loewith R, Stokoe D, Balla A, Toth B, Balla T, Weiss WA, Williams RL, Shokat KM | title = A pharmacological map of the PI3-K family defines a role for p110alpha in insulin signaling | journal = Cell | volume = 125 | issue = 4 | pages=733-47 | year = 2006 | pmid = 16647110 | doi = 10.1016/j.cell.2006.03.035 | url =  }}</ref>
| Pfam = PF00454
| InterPro = IPR000403
| SMART = SM00146
| PROSITE = PDOC00710
| SCOP = 3gmm
| TCDB =
| OPM family =
| OPM protein =
| PDB = {{PDB2|1cja}}, {{PDB2|1e7u}}, {{PDB2|1e7v}}, {{PDB2|1e8w}}, {{PDB2|1e8x}}, {{PDB2|1e8y}}, {{PDB2|1e8z}}, {{PDB2|1e90}}, {{PDB2|1he8}}, {{PDB2|2a4z}}, {{PDB2|2a5u}}, {{PDB2|2chw}}, {{PDB2|2chx}}, {{PDB2|2chz}}
}}
{{Infobox Enzim-lat
| Name = Fosfatidilinozitol 3-kinaza
| EC_number = 2.7.1.137
| CAS_number = 115926-52-8
| IUBMB_EC_number = 2/7/1/137
| GO_code = 
| image = 
| width = 
| caption =
}}
'''Fosfatidilinozitol 3-kinaze''' ({{EC broj-lat|2.7.1.137}}, ''1-fosfatidilinozitolna 3-kinaza'', ''tip III fosfoinozitidna 3-kinaza'', ''Vps34p'', ''tip I fosfatidilinozitolna kinaza'', -{PI}- 3-kinaze ili -{PI3K}-) su familija enzima koji učestvuju u ćelijskim funkcijama poput ćelijskog rasta, proliferacije, diferencijacije, motiliteta, opstanka i intracelularne razmene materije. Sistematsko ime ovog enzima je ''ATP:1-fosfatidil-1D-mio-inozitol 3-fosfotransferaza''.<ref>{{cite journal | title = Synthesis and function of 3-phosphorylated inositol lipids | url = https://archive.org/details/sim_annual-review-of-biochemistry_2001_70/page/535 |author = Vanhaesebroeck, B., Leevers, S.J., Ahmadi, K., Timms, J., Katso, R., Driscoll, P.C., Woscholski, R., Parker, P.J. and Waterfield, M.D. |journal = Annu. Rev. Biochem. |date = 2001 |volume = 70 |pages = 535-602 |pmid = 11395417}}</ref> Ovaj enzim [[Катализа|katalizuje]] sledeću [[hemijska reakcija|hemijsku reakciju]]

: [[Аденозин-трифосфат|ATP]] + 1-fosfatidil-1-{D}--mio-inozitol <math>\rightleftharpoons</math> [[Аденозин-дифосфат|ADP]] + 1-fosfatidil-1-{D}--mio-inozitol 3-fosfat

-{PI3K}- kinaze su familija intracelularnih [[Prenos signala (biologija)|prenosnika signala]] koji imaju sposobnost [[fosforilacija|fosforilacije]] 3 pozicije [[hidroksil]]ne grupe [[inozitol]]nog prstena [[fosfatidilinozitol]]a (-{PtdIns}-).<ref>{{cite web |url=http://www.chem.qmul.ac.uk/iupac/cyclitol/myo.html |title=Myo-inositol |access-date=18. 01. 2011 |archive-url=https://web.archive.org/web/20110806104525/http://www.chem.qmul.ac.uk/iupac/cyclitol/myo.html |archive-date=06. 08. 2011 |url-status=dead |df= }}</ref> Oni su tako]e poznati kao fosfatidilinozitol-3-kinaze. Put koji sadrži [[onkogen]] [[PIK3CA]] i tumor supresor [[PTEN (gen)]] je impliciran u neosetljivost [[tumor]]a na [[insulin]] i [[Insulinu sličan faktor rasta 1|IGF1]].<ref name="urlCell pathway on overdrive prevents cancer response to dietary restriction">{{cite web | url = http://www.physorg.com/news156000728.html | title = Cell pathway on overdrive prevents cancer response to dietary restriction | author = Giese N | date = 11. 3. 2009. | work = | publisher = PhysOrg.com | pages = | language = | archiveurl = | archivedate = | quote = | accessdate = 22. 4. 2009.}}</ref><ref name="pmid19279572">{{cite journal |vauthors=Kalaany NY, Sabatini DM | title = Tumours with PI3K activation are resistant to dietary restriction | journal = Nature | volume = 458 | issue = 7239 | pages=725-31 | year = 2009 | pmid = 19279572 | doi = 10.1038/nature07782 | url = | issn = | pmc = 2692085 }}</ref>

== Otkriće ==
-{PI}- 3-kinaze su otkrili Luis C. Kantli i njegovi saradnici. Do otkrića je došlo nakon identifikacije prethodno nepoznate [[fosfatidilinozitol|fosfoinozitidne]] kinaze vezane za polioma srednji T protein.<ref>{{cite journal |vauthors=Whitman M, Kaplan DR, Schaffhausen B, Cantley L, Roberts TM |title=Association of phosphatidylinositol kinase activity with polyoma middle-T competent for transformation |journal=Nature |volume=315 |issue=6016 |pages=239-42 |year=1985 |pmid=2987699 |doi= 10.1038/315239a0|url=}}</ref> Oni su primetili jedinstvenu supstratnu specifičnost i hromatografske osobine proizvoda ove lipidne kinaze, što je dovelo do otkrića ove fosfoinozitidne kinaze sa izuzetnom sposobnošću da fosforiliše [[fosfatidilinozitol|fosfoinozitide]] u 3' poziciji [[inozitol]]nog prstena.<ref name="Whitman">{{cite journal |vauthors=Whitman M, Downes CP, Keeler M, Keller T, Cantley L |title=Type I phosphatidylinositol kinase makes a novel inositol phospholipid, phosphatidylinositol-3-phosphate |journal=Nature |volume=332 |issue=6165 |pages=644-6 |year=1988 |pmid=2833705 |doi=10.1038/332644a0 |url=}}</ref> Oni su naknadno pokazali da in vivo enzim preferira -{PtdIns(4,5)P2}- kao supstrat, čime se proizvodi fosfoinozitid [[Fosfatidilinozitol (3,4,5)-trisfosfat|-{PtdIns(3,4,5)P3}-]].<ref name="Auger">{{cite journal |vauthors=Auger KR, Serunian LA, Soltoff SP, Libby P, Cantley LC |title=PDGF-dependent tyrosine phosphorylation stimulates production of novel polyphosphoinositides in intact cells |journal=Cell |volume=57 |issue=1 |pages=167-75 |year=1989 |pmid=2467744 |doi= 10.1016/0092-8674(89)90182-7|url=}}</ref>

== Literatura ==
{{reflist|2}}

== Dodatna literatura ==
{{Литература|2}}
* {{Cite journal |vauthors=Vanhaesebroeck B, Leevers S, Ahmadi K, Timms J, Katso R, Driscoll P, Woscholski R, Parker P, Waterfield M |title=Synthesis and function of 3-phosphorylated inositol lipids |url=https://archive.org/details/sim_annual-review-of-biochemistry_2001_70/page/535 |journal=Annu Rev Biochem |volume=70 |issue= |pages=535-602 |year= 2001|pmid=11395417 |doi=10.1146/annurev.biochem.70.1.535}} [http://arjournals.annualreviews.org/doi/abs/10.1146%2Fannurev.biochem.70.1.535]{{Мртва веза}}
* {{Cite journal |vauthors=Schild C, Wirth M, Reichert M, Schmid RM, Saur D, Schneider G |title=PI3K signaling maintains [[Myc|c-myc]] expression to regulate transcription of E2F1 in pancreatic cancer cells |journal=Mol. Carcinog. |volume= 48|issue= 12|pages=1149-58|year=2009 |pmid=19603422 |doi=10.1002/mc.20569 |url=}}
* {{Cite journal |url=http://www.biochemsoctrans.org/bst/037/0615/bst0370615.htm |title=Form and flexibility in phosphoinositide 3-kinases |first5=X |last5=Zhang |first4=WC |last4=Hon |first3=S |last3=Miller |first2=A |last2=Berndt |author=Williams et al. |year=2009 |pmid=19614567 |doi=10.1042/BST0370615 |journal=Biochemical Society Transactions  |volume=37 |issue=Pt 4 |pages=615-626}}
* {{PriceEnzymology3rd}}
* {{TooneAdvancesInEnzymology}}
* {{Branden ProteinStructure2nd}}
* {{SegelEnzymeKinetics}}
{{Литература крај}}
== Spoljašnje veze ==
* {{MeshName|PI-3+Kinase}}
* [http://www.cellsignal.com/pathways/akt-signaling.jsp -{PI3K/Akt}- signalni put]
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{{Kinaze-lat}}

{{Enzimi-lat}}
{{Portal bar-lat|Molekularna i ćelijska biologija|Hemija}}

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